Abstract
Spine gourd (Momordica dioica Roxb.) is originated in Indo Malaya region and widely distributed in tropical and sub-tropical parts of India and adapted to a wide range of soil and climatic conditions (Basumatary et al. 2014). It is highly nutritious vegetable containing high amount of protein as compared to other cucurbitaceous vegetables with a high medicinal value, mainly cultivated for its fruits. Changing conditions had an urge plant breeder to practice on some avant-garde methods such as genetic engineering and mutation breeding, as an addition to traditional methods
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