Abstract
BackgroundInsular function has gradually become a topic of intense study in cognitive research. Recognition memory is a commonly studied type of memory in memory research. GABABR has been shown to be closely related to memory formation. In the present study, we used intellicage, which is a new intelligent behavioural test system, and a bilateral drug microinjection technique to inject into the bilateral insula, to examine the relationship between GABABR and recognition memory.MethodsMale Sprague–Dawley rats were randomly divided into control, Sham, Nacl, baclofen and CGP35348 groups. Different testing procedures were employed using intellicage to detect changes in rat recognition memory. The expression of GABABR (GB1, GB2) in the insula of rats was determined by immunofluorescence and western blotting at the protein level. In addition, the expression of GABABR (GB1, GB2) was detected by RT-PCR at the mRNA level.ResultsThe results of the intellicage test showed that recognition memory was impaired in terms of position learning, punitive learning and punitive reversal learning by using baclofen and CGP35348. In position reversal learning, no significant differences were found in terms of cognitive memory ability between the control groups and the CGP and baclofen groups. Immunofluorescence data showed GABABR (GB1, GB2) expression in the insula, while data from RT-PCR and western blot analysis demonstrated that the relative expression of GB1 and GB2 was significantly increased in the baclofen group compared with the control groups. In the CGP35348 group, the expression of GB1 and GB2 was significantly decreased, but there was no significant difference in GB1 or GB2 expression in the control groups.ConclusionsGABABR expression in the insula plays an important role in the formation of recognition memory in rats.
Highlights
Insular function has gradually become a topic of intense study in cognitive research
By controlling the changes in GABABR expression in the insula, we examined the behavioural changes in recognition memory via intellicage
Reagents Primary antibodies against GB1R and G B2R were purchased from Abcam (Cambridge, UK). GABAB receptor agonist Baclofen and antagonist CGP35348 were obtained from Sigma
Summary
Insular function has gradually become a topic of intense study in cognitive research. Recognition memory is a commonly studied type of memory in memory research. We used intellicage, which is a new intelligent behavioural test system, and a bilateral drug microinjection technique to inject into the bilateral insula, to examine the relationship between GABABR and recognition memory. Recognition memory depends on many memory sub-systems of the brain network, including the visual pathway, temporal lobe medial structure (hippocampus and olfactory cortex) [4], frontal lobe and parietal cortex. Many studies have focused on the role of the medial temporal lobe structure and frontal cortex in recognition memory. The use of stereotactic microinjection technology to study the relationship between the insula and recognition memory is still rare [1]
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