Effects of GABA and glycine receptor antagonists on the activity and PAG-induced inhibition of rat dorsal horn neurons

Abstract

The effects of bicuculline and strychnine on the activity and periaqueductal gray (PAG)-induced inhibition of rat dorsal horn neurons of the lumbar spinal cord were tested. Extracellular single unit recordings were from 36 dorsal horn neurons near a microdialysis fiber passed through the spinal cord for drug application. The GABA, receptor antagonist, bicuculline, was tested on 19 cells, whereas the glycine receptor antagonist, strychnine, was tested on 17 cells. Both bicuculline and strychnine increased the background activity and responses to mechanical stimulation (BRUSH, PRESS, and PINCH) of the skin. They also significantly blocked the PAG-induced inhibition of responses to peripheral mechanical stimuli. This experiment suggests that the mechanism of PAG-induced descending inhibition of dorsal horn neuron activity involves GABA and/or glycine release in the spinal cord and that there is tonic release of these inhibitory neurotransmitters.