Abstract
SummaryFungizone, the deoxycholate complex of Amphotericin B, is widely used in tissue culture media as a precaution against contamination. However, a number of reports indicate that the polyenes are toxic to certain mammalian cell lines at relatively low concentrations and can potentiate the effects of other chemicals. The effects of Fungizone on the growth of cultured V79 Chinese hamster lung fibroblasts were determined: growth was inhibited at concentrations above 10μg/ml (ID50 = 13μg/ml), but Fungizone did not affect cell growth when present at the concentration recommended for use in tissue culture media (2μg/ml). This concentration of Fungizone was found to significantly increase the growth inhibitory effects of four toxicants with different modes of action; however, this effect is not considered sufficient to invalidate the inclusion of Fungizone in media used in cytotoxicity tests on cultured V79 cells.
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