Effects of fructus psoraleae extract on the intestinal absorption kinetics of geniposide and geniposidic acid in rat.

Yan Huo,Erwei Liu,Xiangmei Hou,Yuxing Huang,Lifeng Han,Xiumei Gao,Lei Wang

doi:10.3390/molecules19067557

Yan Huo, Erwei Liu + Show 5 more

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https://doi.org/10.3390/molecules19067557

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Abstract

Cortex Eucommia has been used as a kidney-tonifying herbal medicine with a long history of compatibility with Fructus Psoraleae. Geniposide (GP) and geniposidic acid (GPA) are the two main chemical components in Cortex Eucommia. In the present study, the effects of Fructus Psoraleae extract (FPE) on intestinal absorption kinetics of GP and GPA in rat were investigated. Twenty four male Sprague-Dawley rats were randomly assigned into four groups which were treated with GP, GPA, GP mixed with FPE and GPA mixed with FPE, respectively, by in situ intestinal perfusion for 3 h. The samples of intestinal perfusion solutions were collected every 30 min, and analyzed by ultra high performance liquid chromatography (UPLC). The curves of time and residual quantities of GP and GPA (lnx) in the intestinal perfusion solution and the cumulative absorption rate were obtained. The results showed that FPE exhibited different effects on the intestinal absorption of GP and GPA in rat: it increased the intestinal absorption of GP (p < 0.05), while demonstrated no significant effect on the absorption of GPA.

Highlights

Cortex Eucommia, is widely used in the clinic for the treatment of kidney or muscle weakness, lumbago and fetal irritability [1]
This paper reports for the first time, the effects of Fructus Psoraleae extract (FPE) on the intestinal absorption kinetics of GP and geniposidic acid (GPA)
The results showed that both GP and GPA groups were stable in blank intestinal perfusion solution for 3 h (Table 1)

Summary

Introduction

Cortex Eucommia, is widely used in the clinic for the treatment of kidney or muscle weakness, lumbago and fetal irritability [1]. GP and GPA (Figure 1) are iridoid compounds and active ingredients of Cortex Eucommia and many other Traditional Chinese Medicines [2,3,4,5]. Natural herbal medicines and their single ingredient compounds are widely used as clinical drugs, in Asia. When administered simultaneously, the herbal medicine may significantly influence the absorption pharmacokinetics of the monomer compounds, thereby affect their effectiveness and safety. Several studies [6,7] have investigated the absorption kinetics of GP or GPA, but few studies have assessed the absorption kinetics of these two compounds combined with other Traditional Chinese Medicines

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Discussion

Conclusion