Abstract
The effects of dietary fructose, levamisole and vanadate on insulin-stimulated conversion of d-[U- 14C]glucose to 14CO2 and to 14C-labeled lipid were examined in rat epididymal adipose tissue. Male weanling rats were fed isoenergetic diets containing either 27% (wt/wt) fructose or glucose for 11 wk. During the final 4 wk, rats in each group were either untreated (control) or treated orally with 20 mg/kg body wt levamisole or 0.5 ppm vanadate via their drinking water. Basal glucose oxidation to CO2 was 45% higher in the fructose-control than in the glucose-control group. Insulin-stimulated glucose oxidation in both control groups was not higher than the basal rate in fructose-fed rats. Basal lipogenesis was 31% lower in the fructose-control than in the glucose-control group. Insulin-stimulated lipogenesis was much higher than basal, but was not different between fructose- and glucose-control groups. Levamisole increased basal lipogenesis in fructose-fed rats. Vanadate acted synergistically with fructose in greatly diminishing insulin-stimulated glucose oxidation. Fasting plasma insulin levels were lower and fasting plasma glucose and triglycerides were higher in fructose- than in glucose-fed rats, irrespective of treatment. Results suggest that adaptation to dietary fructose enhances basal oxidative capacity in an insulin-like fashion and reduces the basal lipogenic capacity of adipose tissue. Treatment with levamisole and vanadate lowers the overall rate of glucose metabolism and alters the effects of fructose.
Published Version
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