Abstract
Objective To observe the effects of Frankincense extracts on the biological characteristics of human dermal fibroblasts (FBs) in vitro and explore the possible therapeutic mechanism for wound healing.Methods The optimal concentration of Frankincense extracts was identified by analysing proliferation activity of human normal FBs that treated with different concentration of Frankincense extracts through thiazole blue (MTT) colorimetric assay.Cell cycle,collagen Ⅰ and collagen Ⅲ mRNA levels in FBs treated with the optimal concentration of Frankincense extracts were detected by using flow cytometry (FCM) and real-time polymerase chain reaction (PCR).Results At concentrations of 5 × 10-3 g/L to 5 × 10-2 g/L,the Frankincense extracts significantly enhanced the proliferation of FBs.The most significant concentration was 5 × 10-2 g/L(t =3.89,P ＜0.01),at which an increased percentage of G1 to S and S to G2 phase cells as well as an increased level of collagen Ⅰ and collagen Ⅲ mRNA were obtained compared to the control group.Conclusion Frankincense extracts can notably promote the proliferation of FB and accelerating the cell cycle of FB as well as up-regulating the expression of collagen Ⅰ and collagen Ⅲ,which may enhance the process of wound healing. Key words: Frankincense; Fibroblasts ; Wound healing; Cytoproliferation
Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
