Abstract
To investigate the effect of Foxc1 and Oct4 genes regulated by bone marrow mesenchymal stem cells (BMSCs) transplantation on cardiomyocyte apoptosis after acute myocardial infarction in rats. 180 healthy SD male rats were divided into 3 groups by random number table method, namely control group, acute myocardial infarction (AMI) group and BMSCs group. The rats were sacrificed 4 weeks after transplantation. The TUNEL method was used to detect the cardiomyocyte apoptosis index. The control group basically had no cardiomyocyte apoptosis, while the apoptosis indexes of AMI group (0.67 ± 0.14) was higher than that (0.31 ± 0.12) of BMSCs group (P < 0.05). The OD value of Foxc1 and Oct4 protein in BMSCs group was higher than those in AMI group (all P < 0.05). BMSCs transplantation can reduce cardiomyocyte apoptosis after AMI in rats. The mechanism may be related to the up-regulation of Oct4 and Foxc1 protein expression.
Highlights
Bone marrow mesenchymal stem cells (BMSCs) are derived from bone marrow stromal stem cells and are a subset of cells found in mammalian bone marrow stromal cells with multiple differentiation potentials that differentiate into bone, cartilage, fat, nerve and myoblast (Xiong et al, 2020; Yamada et al, 2019)
Bone tissue can differentiate into osteoblasts and chondrocytes, blood can differentiate into red blood cells, and adipose tissue can differentiate into fat cells (Mahmoud et al, 2020; Dey et al, 2020)
Four weeks after transplanting BMSCs into rats, the immunohistochemical staining of labeled BMSCs showed that the nuclei of positive cells in myocardial tissue located in the non-infarct area were stained brown
Summary
Bone marrow mesenchymal stem cells (BMSCs) are derived from bone marrow stromal stem cells and are a subset of cells found in mammalian bone marrow stromal cells with multiple differentiation potentials that differentiate into bone, cartilage, fat, nerve and myoblast (Xiong et al, 2020; Yamada et al, 2019). A large number of experiments have shown that the myocardial tissue microenvironment can induce differentiation of BMSCs (Chen et al, 2020; Omar et al, 2019; Wu et al, 2017; Zhang et al, 2015). The purpose of bone marrow stem cells in the treatment of myocardial infarction is to reverse the apoptosis of myocardial cells, but the specific mechanism of anti-apoptosis of BMSCs transplanted myocardial infarction disease is still unknown and needs further study. This experiment aims to study the effect of rat bone marrow mesenchymal stem cell transplantation on Foxc and Oct expression and their correlation, by exploring the effect mechanism of bone marrow mesenchymal stem cell transplantation on myocardial cell apoptosis after acute myocardial infarction in rats, it provides a theoretical basis for clinical application of bone marrow mesenchymal stem cell transplantation
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