Effects of four extracts of Chinese herbal medicines on cellular immunity in rats induced by multiple drugs resistant bacillus tuberculosis among pneumoconiosis patients complicated with tuberculosisLU Jun

Abstract

Objective To investigate the regulative effect of the extract of Radix Ranunculi Ternati, Radix Sophorae Flavescenti, Prunella vulgaris L. and Stellera chamaejasme L. on cellular immunity induced by multiple drugs resistant bacillus tuberculosis（MDR-TB） from pneumoconiosis patients complicated with tuberculosis in rats. Methods MDR-TB model in rats was induced by MDR-TB. Normal control group were feed by standard feed, model group were irrigated by normal saline, and the other groups were respectively feed by the extract of Chinese herbal medicines. The content of IFN-y, IL-4, IL-10 and IL-12 were examined by ELISA. RT-PCR was used to detect the mRNA levels of them. Results The content of IFN-y of the four extracts of Chinese herbal medicines were （2.01±0.73）, （1.92±0.56） , （1.98±0.67） and （1.94±0.59） pg/ml, IL-4 were （6.01±1.46）, （6.12±1.35）, （6.47±1.46） and （6.15±1.44） pg/ml, IL-10were （12.09±3.07）, （12.45±4.01）, （12.13±3.43） and （12.54±3.78） pg/ml, IL-12 were （2.99±0.89）, （2.75±0.84）, （ 3.02±0.86） and （ 2.89±0.75 ） pg/ml. Compared to the model group, they resulted in significant in serum IFN-y, IL-12, IL-4 and IL-10 （P〈0.05 or P〈0.01 ） , the mRNA levels of them were significantly （P〈0.05 or P〈0.01 ）. Conclusion The four extracts of Chinese herbal medicines can enhance the cellar immunological function in rats from up-regulation of the level of genetic transcription,accrdingly provide the theory base of healing of pneumociosis patients complicaled with tuberculosis with them. Key words: Radix Ranunculi Ternati; Radix Sophorae Flavescentis; Prunella vulgaris L. ; Stellerachamaejasme L. ; Cytokine