Effects of forkhead box protein M1 down-regulation by RNA interfence on growth,and don and invasion of human ovarian cancer cell

Abstract

Objective To study the effects of RNA interference forkhead box protein M1 (FoxM1) on growth,and clon formation,and invasion of huaman ovarian cancer cell.Methods Using checks The FoxM1 gene mRNA levels were exmined by real-time quantitative polymerase chain reaction (Real-time PCR) method of eight human ovarian cancer cell lines.FoxM1 small interfering RNA (siRNA)were built and synthesized by using chemical methods.The cells were classified into three groups:blank control group (Con-A),the empty plasmid group (Con-B) and FoxM1 siRNA group (siRNA).Among them,the siRNA group was transfected with FoxM1 siRNA.was used to detect cancer cells FoxM1 mRNA and protein levels in each group were detected by Real-time PCR and Western blotting,respectively.The cell viability of each group was measured by cell counting kit-8 (CCK-8) assay.The clones were evaluated by colony formation assay.The invasion ability was evaluated by Transwell method.Results The mRNA levels of FoxM1 gene is highest in the SKOV-3 and HO-8910PM cell lines.After SKOV-3 cell line was transfected by FoxM1 siRNA,the results of the CCK-8 assay showed that the A Values of Con-A,Con-B and FoxM1 group in 72 h were 2.455 ± 0.033,2.442 ± 0.025 and 1.312 ± 0.028,respectively (P ＜ 0.05) ; the results of colony formation assay showed that clones of Con-A,Con-B and FoxM1 group were 100 ±5,93 ±8 and 51 ±3,respectively (P＜0.05) ; the results of the Transwell showed that the membrane cell number of Con-A,Con-B and FoxM1 group were 93 ± 4,86 ± 3 and 36 ± 1,respectively (P ＜0.05).After HO-8910PM cell line was transfected by FoxM1 siRNA,the results of the CCK-8 assay showed that the A Values of Con-A,Con-B and FoxM1 group in 72 h were 2.691 ±0.039,2.560 ±0.033 and 1.455 ± 0.027,respectively (P ＜ 0.05) ; the results of colony formation assay showed that clones of Con-A,Con-B and FoxM1 group were 146 ± 7,145 ± 8 and 85 ± 2,respectively (P ＜ 0.05) ; the results of the Transwell showed that the membrane cell number of Con-A,Con-B and FoxM1 group were 449 ± 15,445 ± 11 and 151 ± 9,respectively (P ＜ 0.05).Conclusion FoxM1 gene might play an important role in growth,proliferation and invasion of human ovarian cancer,and FoxM1 gene may be an important target in human ovarian cancer. Key words: Ovarian cancer; Forkhead box protein M1; Proliferation; Invasion; RNA interfence