Effects of folic acid and sodium selenite on growth performance, nutrient digestion, ruminal fermentation and urinary excretion of purine derivatives in Holstein dairy calves

Abstract

This study evaluated the effects of folic acid (FA) and sodium selenite (SS) supplements on growth performance, nutrient digestion, ruminal fermentation, microbial enzyme activity, microflora and urinary excretion of purine derivatives (PD) in dairy calves. Thirty-six Holstein calves (63 ± 3.4 days of age and 83 ± 2.1 kg of body weight) were randomly assigned to four treatments in a 2 × 2 factorial arrangement. Two levels of FA (0 or 3.0 mg/kg of FA) and SS (0 or 0.3 mg/kg of Se from SS) were added into diets on a dry matter (DM) basis. Nutrient intake and average daily gain increased with FA addition, but were not affected by SS addition. Both supplements increased total tract digestibility of DM, organic matter and neutral detergent fiber. Ruminal pH and ammonia N concentration decreased, but total volatile fatty acids concentration increased with FA or SS addition. Acetate molar proportion and acetate to propionate ratio increased, while propionate molar proportion decreased with FA addition. The unchanged acetate molar proportion, higher propionate molar proportion and lower acetate to propionate ratio were observed with SS addition compared with the control. Activities of xylanase, pectinase, α-amylase and protease as well as populations of total bacteria, protozoa and methanogens were higher for both supplements compared with the control. Carboxymethyl-cellulase activity and Prevotella ruminicola population increased with FA addition. The Ruminobacter amylophilus population decreased with SS addition. The FA×SS interaction was significant for total tract digestibility of crude protein and acid detergent fiber, populations of total fungi, Ruminococcus (R.) albus, R. flavefaciens, Fibrobacter succinogenes and Butyrivibrio fibrisolvens as well as urinary total PD excretion; these variables increased with SS addition and the increase was greater when the diet was not supplemented with FA than with FA supplemented diet. The results indicated that dietary FA and/or SS addition stimulated nutrient digestion, ruminal fermentation, microbial growth and urinary total PD excretion.