Effects of fluoride and epigallocatechin gallate on soft-drink-induced dental erosion of enamel and root dentin.

Abstract

Fluoride and epigallocatechin gallate (EGCG) have been proven to prevent dental caries. The purpose of this study was to evaluate the effects of fluoride and EGCG on soft-drink-induced dental erosion invitro. Forty enamel and dentin specimens were prepared from extracted human teeth. The specimens were divided into 4 groups and treated separately with distilled water (as control), 0.5M sodium fluoride (NF), 400μM EGCG (EG), and a solution containing 0.5M NaF and 400μM EGCG (FG). Cyclic erosive treatment was performed according to the experimental procedures. The specimens were analyzed using laser scanning confocal microscopy, scanning electron microscopy, and a microhardness tester. The data were analyzed using ANOVA and Bonferroni's post hoc test. The significance level was set at 5%. The amount of substance loss was lower in the NF and EG groups than in the control group (p<0.05). The erosion-caused substance loss was more pronounced in the dentin than in the enamel specimens. Surface microhardness loss was lower in the NF and EG groups than in the control group (p<0.05). The diameter of the dentinal tubule was wider in the control group than in the NF and EG groups (p<0.05). No combined effects were observed in the FG group. Both fluoride and EGCG are effective in preventing soft-drink-induced erosion compared with the control group. Fluoride and EGCG may interfere with each other. The mechanisms of the anti-erosive effect need to be explored in the future.