Abstract
We examined the effects of daily inflorescence size (three-, six-, nine- and 12-flowered) and the position of flowers within an inflorescence (bottom, middle and top) on the frequency of self-fertilization using genetic markers and experimental manipulation of garden populations of Eichhornia paniculata, a self-compatible bee-pollinated plant. Based on the observed tendency for bees to forage upwards on inflorescences and a model of the relation between pollen carry-over and the number of flowers visited per inflorescence, we predicted that the frequency of self-fertilization should increase from bottom to top flowers and with increasing inflorescence sizes. Electrophoretic analysis of open-pollinated progeny arrays supported both of these predictions
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