Effects of flooding on carbohydrate and ABA levels in roots and shoots of alfalfa

Abstract

ABSTRACTAlfalfa is sensitive to waterlogging, and its yields are significantly reduced under this condition. We investigated the effects of soil flooding on free abscisic acid (ABA) accumulation in shoots and roots of alfalfa in relation to plant growth and stomatal conductance responses. The production of dry matter in alfalfa was significantly affected by flooding mainly as a result of a rapid reduction in root growth. Shoot dry matter accumulation was maintained during the first 10 d of treatment and started to decline thereafter. Foliar concentration of the major mineral elements (N, P, K) was reduced by flooding, whereas only K concentration decreased in roots of flooded plants. Regrowth declined with duration of flooding and was less than 50% of controls after 2 weeks. While no changes in ABA concentration could be detected in flooded roots, an increase was noted within a few days in leaves when compared to unflooded controls. This increase in free ABA coincided with the accumulation of large quantities of starch in leaves and a rapid decline in leaf stomatal conductance. Our results support the suggestion that leaf ABA originates from the leaf itself and may be accumulating along with starch as a result of reduced translocation to the roots. Our observation of large accumulations of sucrose in flooded roots agrees with previous reports that supply of carbohydrates is not a limiting factor to root anaerobic metabolism in flooded alfalfa.