Effects of Flavonoids from Potamogeton crispus L. on Proliferation, Migration, and Invasion of Human Ovarian Cancer Cells.

Yuanda Du,Jinhong Feng,Jian Liu,Renqing Wang,Haijie Zhang,Jung Weon Lee

doi:10.1371/journal.pone.0130685

Yuanda Du, Jinhong Feng + Show 4 more

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https://doi.org/10.1371/journal.pone.0130685

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Journal: PloS one	Publication Date: Jun 22, 2015
Citations: 15	License type: CC BY 4.0

Affiliation: Shandong Academy of Sciences, Shandong University

Abstract

In order to explore the efficient utilization of plant resources from constructed wetlands, the potential anti-metastatic effects of flavonoids from Potamogeton crispus L. were investigated in human ovarian cancer cells (ES-2). Two major flavonoids, luteolin-3ʹ-O-β-D-glucopyranoside and flavone-6-C-β-D-glucopyranoside, were isolated from P. crispus and identified. The effects of these flavonoids on cell proliferation, cell morphology, cell cycle, apoptosis, and cell migration and invasion were then investigated. Furthermore, reverse transcriptase polymerase chain reaction assays and western blotting analysis were conducted to examine the expression level of mRNA and protein. Results indicated that Luteolin-3ʹ-O-β-D-glucopyranoside inhibited ES-2 cell migration and invasion and suppressed the expression of two matrix metalloproteinases (MMPs), MMP-2 and MMP-9, and Flavone-6-C-β-D-glucopyranoside had no significant inhibitory effects on ES-2 cells. Thus, this study demonstrated the potential anti-metastatic properties of a P. crispus flavonoid, and provided a scientific approach for the screening of promising natural resources from constructed wetlands to identify useful products for use in the pharmaceutical and healthcare industries.

Highlights

Plants play a key role in the construction of engineered wetland environments and they should be managed strictly to maintain wetland efficiency while minimizing the risk of secondary pollution and negative ecological effects on the ecosystem
The ethanol extract was suspended in water before partitioning with petroleum ether (PE), ethyl acetate (EtOAc), and n-butanol sequentially; these were concentrated under a vacuum to give a PE extract, an EtOAc extract, and an n-butanol extract
high-performance liquid chromatography (HPLC), nuclear magnetic resonance (NMR), and HR-ESI-MS were conducted to isolate and identify the two main compounds within the P. crispus EtOAc extract. These compounds were identified and characterized as Luteolin-30-O-β-d-glucopyranoside (LU30O-GP) and Flavone-6-C-β-dglucopyranoside (FL6C-GP) by comparing their spectral data (HPLC, HR-ESI-MS, 1H and 13C NMR) and physicochemical properties with those reported in the literature [23,24,25]

Summary

Introduction

Plants play a key role in the construction of engineered wetland environments and they should be managed strictly to maintain wetland efficiency while minimizing the risk of secondary pollution and negative ecological effects on the ecosystem. Efficient utilization of high-biomass wetland plant resources is important because it encourages harvesting and sustainable management of constructed wetlands. Potamogeton crispus L. is one of the most important plants employed in constructed wetland ecosystems [1]. Previous reports have identified carotenoids, fatty acids, lignan, labdane diterpenoids, flavonoids, and phytosterins in P. crispus [2,3,4,5], and carotenoid extracts from P. crispus were reported to induce apoptosis in HeLa cells in vitro [6].

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