Abstract
Eleutherococcus sieboldianus (Makino) Koidz. is a local product from the area in and around Yonezawa City in Yamagata Prefecture, Japan. It has been used as a medicinal plant for a long time. We isolated and identified four types of flavonoid glycosides [astragalin (1), isoquercetin (2), rhamnocitrin 3-O-glucoside (3), and nicotiflorin (4)], a triterpene [methyl hederagenin (5)], and three types of triterpene glycosides [δ-hederin (6), echinocystic acid 3-O-arabinoside (7), and cauloside B (8)] from the methanol extract of E. sieboldianus, which regulates lipid accumulation in 3T3-L1 preadipocytes. Among the compounds isolated, 2 and 8 up- and down-regulated lipid accumulation and insulin induced adipocyte differentiation in 3T3-L1 preadipocytes. Compound 2 induced up-regulation of lipid accumulation and decreased adipocyte size, while 8 down-regulated lipid accumulations without decreasing cell size. Additionally, 2 increased adipogenic proteins [peroxisome proliferator-activated receptor γ (PPARγ), CCAAT/enhancer-binding protein alpha (C/EBPα), and fatty-acid-binding protein 4 (FABP4)]. In contrast, 8 decreased the levels of all adipogenic proteins and glucose transporter type 4 (GLUT4), but increased adiponectin.
Highlights
Eleutherococcus sieboldianus (Makino) Koidz. is a local product from the area in and around Yonezawa City in Yamagata Prefecture, Japan
The effects of four types of flavonoid glycosides, three types of triterpene glycosides, and a triterpene isolated from E. sieboldianus on 3T3-L1 cells were evaluated, and this study found that a flavonoid glycoside and a triterpene glycoside exhibited anti-diabetic and anti-obesity effects respectively
The cytotoxicity and the regulation of intracellular triglycerol levels by these extracts are shown in Figures 1 and 2
Summary
Eleutherococcus sieboldianus (Makino) Koidz. (fiveleaf aralia) is a local product from the area in and around Yonezawa City in Yamagata Prefecture, Japan. E. sieboldianus has been used as a medicinal plant for preventing cancer and cardiovascular diseases and it is regarded as a longevity drug [1]. E. sieboldianus has been used as a traditional Korean medicine (min gal pi) [2]. Constituents from E. sieboldianus have not been elucidated, except for saponins [3]. Molecules 2017, 22, 671 biological activities of crude extracts of E. sieboldianus have been reported, including anti-oxidant, anti-diabetic, anti-obesity, and anti-microbial properties, and the lowering of blood lactate for the usage of functional food [1,4,5,6]. We were interested in identifying the anti-diabetic and anti-obesity components from E. sieboldianus, because treating these diseases will contribute to improving human health
Sign-in/Register to view highlights & summary Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
