Effects of Flavin-Goethite Interaction on Goethite Reduction by Shewanella decolorationis S12.

Gang Zhao,Enze Li,Jianjun Li,Xunan Yang,Meiying Xu,Fei Liu

doi:10.3389/fmicb.2019.01623

Gang Zhao, Enze Li + Show 4 more

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https://doi.org/10.3389/fmicb.2019.01623

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Abstract

Flavin mononucleotide (FMN) and riboflavin are structurally similar flavins, except for the presence of a phosphate group on the FMN molecule. They are used by a variety of electroactive bacteria as extracellular electron shuttles in microbial Fe reduction and inevitably interact with Fe (hydr)oxides in the extracellular environment. It is currently unknown whether flavin/Fe (hydr)oxide interaction interferes with extracellular electron transfer (EET) to the mineral surface. In this study, we found that the goethite reduction rate was lower when mediated by FMN than by RF, suggesting that FMN was less effective in shuttling electrons between cells and minerals. Nevertheless, the phosphate group did not prevent the FMN molecule from accepting electrons from bacterial cells and transferring electrons to the mineral. Results of adsorption experiment, attenuated total reflectance (ATR) Fourier transform infrared (FTIR) spectroscopy, and bacterial attachment trend analyses showed that FMN exhibited strong adsorption on goethite surface by forming phosphate inner-sphere complex, which prevented bacterial cells from approaching goethite. Therefore, the interaction between FMN and goethite surface may increase the distance of electron transfer from bacterial cells to goethite and result in lower EET efficiency in comparison to those mediated by riboflavin. To our knowledge, these data reveal for the first time that the interaction between flavin and Fe (hydr)oxide affect flavin-mediated electron transfer to mineral surface and add a new dimension to our understanding of flavin-mediated microbial Fe reduction processes.

Highlights

Microbial reduction of insoluble Fe(III)oxides to soluble Fe(II) form is a ubiquitous redox process in natural environments (Weber et al, 2006; Byrne et al, 2015; Venkidusamy et al, 2018)
When goethite reacted with Flavin mononucleotide (FMN), the zeta potential decreased from −45.898 ± 1.327 to −56.841 ± 3.393 mV
In the systems without bacteria cells, the concentration of FMN and RF did not change over time, indicating that the reduction processes were caused by bacteria cells

Summary

Introduction

Microbial reduction of insoluble Fe(III) (hydr)oxides to soluble Fe(II) form is a ubiquitous redox process in natural environments (Weber et al, 2006; Byrne et al, 2015; Venkidusamy et al, 2018). 2008; Byrne et al, 2015) They can transfer electrons to the mineral surface by direct contact or by using flavins [e.g., flavin mononucleotide (FMN) and riboflavin] as extracellular electron shuttles (von Canstein et al, 2008; Shi et al, 2016). The latter approach has been shown to be faster for reduction of Fe(III) (hydr)oxides (von Canstein et al, 2008; Ross et al, 2009; Huang et al, 2018). Low concentrations of FMN and riboflavin have been detected in pore water and water column profiles from a coastal marine basin, suggesting that FMN and riboflavin may be common components of extracellular Fe(III) reduction in natural environments (Monteverde et al, 2018)

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