Effects of Flammulinavelutipes Stem Base on Microflora and Volatile Fatty Acids In Caecum of Growing Layers under Heat Stress Condition

S Wang,H Song,S Mahfuz

doi:10.1590/1806-9061-2019-0989

Abstract

ABSTRACT The present study was undertaken to investigate the effects of Flammulinavelutipes stem base (FVS) on growth performance, microbial flora and volatile fatty acids of growing layers under heat stress condition. A total of 72 ISA Brown hens were randomly divided into six treatments: thermoneutral temperature control group (CON), heat stress control group (HS), heat stress antibiotic group (ANT) as positive control and heat stress FVS groups (20, 40 or 60 g/kg FVS). The experimental period had a duration of 28 d (days 84-112). On day 98, daily gain average was significantly higher (p<0.05) in the FVS groups than in the HS group. The number of bands in the FVS groups were higher (p<0.05) than in the HS group on day 98. The microbial similarity between the 60 g/kg FVS group and the HS group were the lowest on day 98. FVS group’s specific bacteria were mainly Coprococcus comes, [Clostridium] papyrosolvens, Butyricicoccuspullicaecorumon day 98. Whereas on day 112, the FVS groups specific bacteria were mainly Parabacteroides distasonis, Coprobacterfastidiosus, Elusimicrobiumminutum. The content of acetic acid and butyric acid were higher (p<0.05) in 20 g/kg FVS group than in the CON group on day 112. In conclusion, FVS can lighten the adverse effect of heat stress by increasing the diversity of intestinal flora in growing layers.

Highlights

The condition of the gut system affects the nutrients utilization for organ development, tissue growth and immune system maturation in the host
This study examined the effect of Flammulinavelutipes stem base (FVS) on growth performance, caecal microflora and Volatile fatty acids (VFA) concentration in ISA Brown growing layers under heat stress condition
The average daily gain (ADG) in the FVS groups had no significant difference with the control group (CON) group and antibiotic group (ANT) group in the whole period of the test

Summary

Introduction

The condition of the gut system affects the nutrients utilization for organ development, tissue growth and immune system maturation in the host. Cecal microbial populations are the indication of gut health in animals (Mahfuz et al, 2017). The cecum is a complex ecosystem of microbial colonization in poultry. Volatile fatty acids (VFA) usually is produced through bacterial fermentation in the cecum that is necessary for the intestinal function and intestinal integrity (Meimandipour et al, 2010). High-through put sequencing has been used by many researchers to investigate the gut microbial diversity in animals (Wang et al, 2017). Intestinal flora has an important influence on host health (Chang et al, 2016). The composition and activities of intestinal microflora can be altered by dietary patterns, such as feed additives (Maesschalck et al, 2015) and antibiotics (Kalter et al, 2010; Zou et al, 2016)

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Discussion

Conclusion