Effects of fine particulate matter on cell vitality and beta 1 adrenergic receptor expression in H9c2 myocardial cells

Abstract

Objective: To investigate the effects of fine particulate matter on cell vitality and beta 1 adrenergic receptor expression in H9c2 myocardial cells. Methods: During the period Dec 2010 through Mar 2011, fine particulate matter was collected from areas. H9c2 myocardial cells were exposed to various doses of fine particulate matter (1 μg/mL, 10 μg/mL and 100 μg/mL), either water-soluble or water-insoluble, for 24 h. The morphology of H9c2 cell was examined by optical microscope; cell viability was check by using MTT assay, expression of beta 1 receptor was determined by Western blot. Results: H9c2 myocardial cell survival rate in 1 μg/mL, 10 μg/mL and 100 μg/mL water-soluble particulate matter group was (95.0±2.5)%, (89.0±2.3)%, and (84.0±2.8)%, otherwise in the water-insoluble particulate matter group was (95.8±2.5)%, (82.6±2.2)%, and (57.7±2.0)%. The difference between the 100 μg/mL group and the control group was statistically significant (P<0.05). The expression of beta 1 receptor of H9c2 myocardial cell in 1 μg/mL, 10 μg/mL and 100 μg/mL water-soluble particulate matter group was 1.08±0.06, 1.27±0.05, 1.71±0.10, but in the water-insoluble particulate matter group was 1.30±0.05, 2.28±0.14, 5.70±0.23. The difference between the 100 μg/mL group and the control group was statistically significant (P<0.05). With particulate matter concentrations heighten, beta 1 receptor mRNA express heighten. Under the same concentration, the water insoluble composition induced beta 1 receptor expression more obviously (P<0.05). Conclusion: Fine particulate matter can decrease the cell viability and increase beta 1 receptor mRNA expression in myocardial cell.