Effects of FGF-1 and FGFR1 on the Genesis of Spontaneous Breast Cancer in TA2 Mice

Abstract

Objective:To compare the expression of FGF-1 and FGFR1 in normal breast tissues,precancerous breast tissues and breast cancer tissues of TA2 mice,and to discuss the effect of FGF-1 and FGFR1 on the genesis of spontaneous breast cancer in TA2 mice.Methods:Normal breast tissues were obtained from normal TA2 female mice(12 cases).Precancerous breast tissues and breast cancer tissues were obtained from TA2 female mice with spontaneous breast cancer(17 cases).Immunohistochemistry was used to detect the expression of FGF-1,PCNA,and cyclin D1 proteins.Real time-PCR was employed to observe the expression of FGF-1 and FGFR1 mRNA.Results:The expression of FGF-1 mRNA and protein was higher in breast cancer and precancerous lesions than in the normal breast tissues(P0.05).No significant difference was found in FGF-1 expression between breast cancer tissues and precancerous lesions(P0.05).The expression of FGFR1 mRNA was higher in breast cancer tissues and precancerous lesions than in the normal breast tissues(P0.05).A significant difference was found in FGFR1 mRNA expression between breast cancer tissues and precancerous lesions(P=0.046).The expression of PCNA and cyclin D1 was higher in breast cancer tissues and precancerous lesions than in the normal breast tissues(P0.05).The expression of cyclin D1 was higher in breast cancer tissues than in precancerous lesions(P=0.001).Conclusion:FGF-1 and FGFR1 may contribute to the genesis of spontaneous breast cancer in TA2 mice by regulating cell cycle and promoting cell proliferation.