Effects of ferulic acid on proliferation, invasion and apoptosis of osteosarcoma cells

Abstract

Objective To explore the therapeutic effect of ferulic acid on osteosarcoma and its specific therapeutic mechanism. Methods Osteosarcoma 143B cell lines were divided into 4 groups. The cells treated with 50 mmol/L ferulic acid were the low dose group, the cells treated with 100 mmol/L ferulic acid were the middle dose group, and the cells treated with 200 mmol/L ferulic acid were the high dose group. Cell viability was measured by methyl thiazol tetrazolium (MTT), apoptosis and cell cycle were detected by flow cytometry, and B lymphoma-2 gene (bcl-2), bcl-2 related X protein (bax), cyclin-dependent kinase 2, 4, 6 (CDK2, CDK4, CDK6) were detected by Western blotting. The expression of CDK2, CDK4, CDK6. Finally, the invasive ability of each group was detected by Transwell. Results After treatment with ferulic acid, the apoptosis rate of 143B cells was significantly increased, the survival rate was significantly decreased, the cell invasion inhibition ratewas significantly increased [control group 0%, low dose group (23.14±2.85)%, medium dose group (50.26±3.73)%, high dose group (72.15±4.58)%, P<0.05], G0/G1 phase cell ratio was significantly increased [control group (40.52±6.58)%, low dose group (52.26±5.72)%, middle dose group (70.26±6.85)%, high dose group (82.98±8.52)%, P<0.05], the expression of bcl-2, CDK2, CDK4, CDK6 was significantly decreased, and the expression of bax protein was significantly increased, and these changes were dose-dependent with ferulic acid. Conclusion Ferulic acid can treat osteosarcoma by regulating the apoptosis of osteosarcoma cells, which is dose dependent. Key words: Ferulic acid; Osteosarcoma; Apoptosis