Effects of feeding endophyte-infected tall fescue seed on reproductive performance in male CD-1 mice by competitive breeding

Abstract

The objective of this study was to assess the effects of endophyte-infected tall fescue (KY-31) seed (80% infected, containing Acremonium coenophialum ) on the reproductive performance of male CD-1 mice measured by competitive breeding. Sixteen mature CD-1 male mice were randomly allocated to one of two dietary treatments containing 50% mouse chow and either 1) 50% noninfected tall fescue seed, restricted to the daily feed intake of dietary treatment 2, or 2) 50% infected tall fescue seed (w/w) fed ad libitum. Mice were preconditioned on their respective diets for 50 d prior to 96 h of cohabitation with 10 mature CD-1 females. Following removal of males, the females were carried to full term. Males were sacrificed, and testicular, epididymal weights, sperm motility and progressive sperm motility were assessed. The results showed no differences (P>0.05) in average daily weight gain (ADG) and body weight changes during the course of the experiment between the two dietary treatments. There were significant reductions (P<0.05) in total testes weight (0.238 vs 0.214 g), epididymal weight (0.087 vs 0.073 g), sperm motility (82.5 vs 58.7%), and progressive motility (3.7 vs 2.6; scale 0 to 4) between dietary treatments 1 and 2, respectively. Similarly, significant differences in pregnancy rates were 53.8 and 45.0%, and the average numbers of pups born per litter were 10.67 and 9.84, respectively. These data suggest that 50% endophyte-infected fescue seed in the diet of male CD-1 mice can affect their reproductive capacity by influencing the testicular-epididymal weights, and subsequently, the quality of spermatozoa and overall fecundity.