Effects of extrusion pressure and printing speed of 3D bioprinted construct on the fibroblast cells viability

Abstract

In 3D bioprinting system, there are varies set of printing parameter involved that will affect the structure of bioprinted construct and cell viability. This study reports the effect of extrusion pressure and printing speed on the structure of construct and cell viability. This study contains two parts which first, to see the effect of the morphological structure of the construct by printing using Pluronic F127 and second, too evaluate the viability of the cell on the bioprinted construct by printing using fibroblast cell-laden Lithium phenyl-2,4,6-trimethylbenzoylphosphinate (LAP) and Gelatin Methacrylate (GelMa). The construct was printed by using extrusion based 3D bioprinter with various ranges of extrusion pressure and printing speed. Based on the experiment, the result shows that printing at lower extrusion pressure requires a lower printing speed whereas printing at higher extrusion pressure requires a higher printing speed. For the cell viability of the bioprinted construct, printing at higher extrusion pressure will reduce the percentage of cell viability.