Effects of Extract of Arrabidaea chica Verlot on an Experimental Model of Osteoarthritis.

Cleydlenne Costa Vasconcelos,Fernando César Vilhena Moreira Lima,Emerson Lucas Frazão Sousa,Maria Do Socorro De Sousa Cartágenes,João Batista Santos Garcia,Cláudia Quintino Da Rocha,Darleno Sousa Camelo,Alberto Jorge Oliveira Lopes,Gyl Eanes Barros Silva

doi:10.3390/ijms20194717

Cleydlenne Costa Vasconcelos, Fernando César Vilhena Moreira Lima + Show 7 more

Open Access

https://doi.org/10.3390/ijms20194717

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Abstract

The aim of this study was to analyze the analgesic potential of Arrabidaea chica extract (EHA) as an alternative to osteoarthritis (OA) treatment. Thus, the extract was initially evaluated by the cyclooxygenase inhibition test. The analgesic effect of the extract, in vivo, was also verified in a model of OA induced by sodium monoiodoacetate (2 mg). EHA was administered to rats at doses of 50, 150, and 450 mg/kg between 3 and 25 days after OA induction. The animals were clinically evaluated every 7 days, euthanized at 29 days, and the liver, spleen, kidney and knee collected for histopathological analysis. The chemical composition of EHA was identified by HPLC-MS and the identified compounds submitted to molecular docking study. The results showed that the extract promoted cyclooxygenase inhibition and produced significant improvements in disability, motor activity, hyperalgesia, and OA-induced allodynia parameters, in addition to improvements in the radiological condition of the knees (but not observed in the histopathological study). Chemically the extract is rich in flavonoids. Among them, we evidence that amentoflavone showed very favorable interactions with the enzyme COX-2 in the in silico analysis. Thus, it is concluded that A. chica has important analgesic properties for the treatment of OA.

Highlights

The plants have contributed significantly to the development of new therapeutic strategies for the treatment of several diseases, mainly through the secondary metabolites, that can interfere in the performance of mediators of the inflammatory process, second messenger production, and expression of transcription factors [1,2,3]
Since many studies have proven its therapeutic potential, including its anti-inflammatory activity [36,37,38], and due to the fact that OA is a disease in which the inflammatory process plays a central role in the pathogenesis [8], we evaluated this plant against OA
The results of the clinical evaluations of the present study show that A. chica extract 450 mg/kg, from day 21 of treatment, significantly reduced allodynia, and for all doses on day 28 [42] (Figure 5)

Summary

Introduction

The plants have contributed significantly to the development of new therapeutic strategies for the treatment of several diseases, mainly through the secondary metabolites, that can interfere in the performance of mediators of the inflammatory process, second messenger production, and expression of transcription factors [1,2,3]. Bonpl.) B.Verlot (Bignoniaceae) plant species used in this study has been distinguished by its pharmacological potential, associated mainly to its antioxidant, astringent, antimicrobial, antitumor, and anti-inflammatory activities [4,5]. Osteoarthritis (OA) is the disease in which the inflammatory process plays a critical role in its pathogenesis [6]. A. chica could represent a potential alternative to treatment.

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