Abstract
The aim of this study was to determine the effects of extracellular Ca 2+ concentration ([Ca 2+] e) on phagocytosis and intracellular Ca 2+ concentration ([Ca 2+] i) in bovine polymorphonuclear leukocytes (PMNs). The experiments were performed by using blood samples from parturient paretic and clinically normal parturient cows and manipulating the [Ca 2+] e in vitro. Phagocytosis by PMNs (with and without stimulation with phorbol myristate acetate and inhibition with cytochalasin B) and resting [Ca 2+] i were significantly lower in parturient paretic cows. Repletion of Ca 2+ in the extracellular media for the samples from these animals increased phagocytosis and resting [Ca 2+] i. In the blood of clinically normal parturient cows, decreasing the [Ca 2+] e decreased phagocytosis and resting [Ca 2+] i in PMNs, but increasing the [Ca 2+] e did not affect phagocytosis. These results suggest that the hypocalcemic condition of parturient paretic cows in vivo causes decreased phagocytosis and resting [Ca 2+] i in PMNs, which may partly contribute to greater susceptibility to infection.
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