Effects of expression of calpain mRNA in rabbits exposed to vibration by hind legs

Abstract

To study the effects of expression of calpain mRNA in rabbits exposed to vibration by hind legs. 32 New Zealand rabbits were randomly divided into a control group and 3 experimental groups according to 4-hour energy-equivalent frequency-weighted acceleration[ahw (4)]: low (4.33 m/s(2)) , moderate (8.67 m/s(2)) and high (17.34 m/s(2)) intensity group to accepted the vibration by hind legs. 45 ds later, brain and skeletal muscle tissue of rabbits were taken to detect the expression of calpain-1 and calpain-2 mRNA by RT-qPCR technique. The relative content of calpain-1 mRNA in the brain tissues in rabbits of low, medium and high intensity group were 8.35±3.75,9.64±4.54,5.10±5.26. While the relative content of calpain-2 mRNA in the brain tissues in rabbits of low, medium and high intensity group were 7.34±4.97,8.50±5.66, 8.16±5.59. Compared with the control group (1.10±0.29, 0.56±0.43) , the expression of calpain-1 and calpain-2 mRNA of the intensity groups showed an significantly increasing trend (P<0.01). In skeletal muscle tissue, the relative content of calpain-1 mRNA were 4.36±2.05, 7.37±4.06, 12.46±6.21.Compared with the control group (0.98±0.59) , the expression of calpain-1 mRNA of experiment groups were significantly higher (P<0.05) .The expression of calpain-2 mRNA of the intensity groups had no significantly difference with the control group (P>0.05). The expression of calpain-1 and calpain-2 mRNA can be promoted by the vibration by the hind legs.