Abstract
AS the main intracellular receptor of ca2+ , calmodulin (CaM) , which is extensively distributed in various types of eukaryotic cells, is a multifunctional protein regulating the cell growth, differentiation and transformation''] . Since the antisense RNA of CaM can more specifically inhibit CaM and has no pharmacological toxicity to cells compared with most of the antagonists of CaM, we constructed human hepatoma cell lines expressing CaM antisense RNA and analyzed their characteristics of growth, distribution of cell cycle, gene expression and the relationship with transformation under low levels of CaM in order to explore the effects of CaM on cell proliferation and transformation. 1 Materials and methods 1.1 Bacteria strains, cell lines and their culture E . coli HI310 1, E . coli DH5 a and E . coli DH1 were used and cultured12 I . Human hepatoma 7402 cell line and the established cell lines were cultured in DMEM (GEBCO) supplemented with 10 % fetal bovine serum ( FBS) (Tianjin Blood Research Institute) at 37°C , 5 % CQ . * To whom correspondence should be addressed.
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