Abstract
The effects of exposure of boar spermatozoa to bovine follicular fluid (bFF) on freezability were examined. It was found that exposure to 75% bFF at 25°C for 1h before freezing yielded high post-thaw motility, progressive motility and velocity at 3h after thawing. But longer periods of bFF exposure did not further increase the aforementioned parameters. The post-thaw progressive motility was significantly higher in spermatozoa exposed to various concentrations of bFF than that of the control group immediately after thawing. All the parameters at different concentrations of bFF treatments were significantly higher than those of the control group at 3h of incubation after thawing. These observations suggest that exposure to bFF before freezing may increase the resistance of boar spermatozoa to cryopreservation stress. It is of great interest to elucidate the mechanism by which bFF protects spermatozoa from cryoinjury.
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