Effects of exogenous solution treatment on germination, antioxidation and flavonoid biosynthesis of Tartary buckwheat (Fagopyrum tataricum (L.) Gaertn.)

Abstract

Exogenous solution stress treatment is the most common treatment method in the study of Tartary buckwheat (Fagopyrum tataricum) germination. The effects of caffeic acid, L-Phe and NaCl on differentially expressed metabolites (DEMs) and their metabolic pathways during Tartary buckwheat germination were studied by non-targeted metabolomics. It was found that the germination characteristics and antioxidant capacities of Tartary buckwheat were significantly increased after germination with T4 (caffeic acid 17 mg/L, L-Phe 2.7 mmol/L, NaCl 2.7 mmol/L) treatment. Compared with other groups, DEMs in T4 were mainly concentrated in the metabolic pathways of phenylalanine metabolism and flavonoid biosynthesis. That was because Tartary buckwheat germinated under T4 treatment completed the synthesis and transformation of related compounds in the metabolic pathway by up-regulating the metabolites of phenylpropanoid biosynthesis and flavonoid biosynthesis, making the body adapt to stress and promote the enrichment of flavonoids at the same time. This study provided a theoretical basis for exploring exogenous solution to stimulate Tartary buckwheat germination, stress adaptation and flavonoid accumulation.