Effects of Exogenous SA and H2O2 on Wheat-Seedling�s Antioxidant Enzymes in Different Temperatures

Baoting Fang,Simeng Du,Cheng Yang,Junqin Yue,Deqi Zhang,Hanfang Wang,Xiangdong Li,Yanjing Wang,Feng Qin,Yunhui Shao

doi:10.37358/rc.20.6.8216

Abstract

In order to clarify the compensation effect of exogenous salicylic acid (SA) and H2O2 on wheat growth, this paper, by setting different growth temperatures in the artificial climate chamber, has studied the effects of antioxidant enzymes and proline content of wheat seedlings with spraying SA, hydrogen peroxide (H2O2) and SA+H2O2. The results show that catalase (CAT) activity, in 0 and 5 �C is lower than 10 and 15 �C, while the peroxidase (POD) activity is opposite. The activity of ascorbate peroxidase (APX) increases gradually with temperature increasing. Exogenous SA, H2O2, and SA+H2O2 could effectively improve the activity of wheat antioxidant enzymes and the content of proline (Pro), while the SA+H2O2 treatment improves obviously.

Highlights

Wheat is one of the main food crops in China
At the time of 48 h, except that salicylic acid (SA) in 0 °C is slightly higher than CK, other treatments in 0 and 5 °C are lower than CK, and the enzyme activity of SA+H2O2 significantly reduce in 5 °C
Under the conditions of 10 and 15 °C, except that the 10°C H2O2 treatment is slightly lower than CK, the other treatments are all higher than CK, and the enzyme activity of SA+H2O2 treatment increase significantly

Summary

Introduction

Wheat is one of the main food crops in China. The production has been affected by extreme weather in recent years, especially in northern of China. The research about tobacco, which was treated with SA in 4°C for 4 days, shows that the activity of SOD, CAT, POD and APX increased [2, 3]. Anna Ignatenko et al [5] treated winter wheat with exogenous SA, and found that, in 4 °C, exogenous SA could increase the activity of SOD, CAT, POD and the content of Pro, but the content of MDA and H2O2 firstly increased and decreased. [14, 15] shows that exogenous H2O2 could improve rape seed germination rate, promote CAT POD enzyme activity, so as to enhance its cold resistance, while Liu [16] pointed out that H2O2 might be located in the upstream of ABA, by controlling its expression, which could alleviate cell membrane damage in low 5 °C. In different studies, there are few studies on wheat, especial the combined effect of SA and H2O2 on the wheat fewer

Methods

Results

Conclusion