Abstract
In the present study, the effects of indole-3-butyric acid (IBA) alone and in combination with myo-inositol under in vitro conditions on morphogenic and biochemical responses in the MxM 14 (Prunus avium × Prunus mahaleb) sweet cherry rootstock were investigated using shoot tips as explants. The best treatment that induced the better response for rooting in terms of root number per rooted explant (16.46), root length (23.12 mm), root fresh weight (0.372 g), root dry weight (0.037 g) and rooting percentage (100 %) was 9.84 μΜ IBA in the absence of myo-inositol. Thus, myo-inositol suppressed the positive effect of IBA on rooting. The simultaneous application of IBA and myo-inositol achieved the better response for shoots development regarding their length (22.5–25.6 mm) in relation to the control (15 mm). However, the optimum shoot biomass was obtained with 9.84 μΜ IBA resulting in a 4-fold increase in shoot fresh (0.455 g) and dry weight (0.045 g) compared to the control. Regarding biochemical parameters, it was shown that there is no evident relation between chlorophyll levels, endogenous leaf proline or carbohydrate content in both leaves and roots, and improvement of rooting and shoot biomass in the respective observed treatments. On the contrary, the enhancement of rooting and shoot biomass could be related to the elevated endogenous proline levels in roots. According to the results obtained, there may be a correlation between the augmented proline levels in both leaves and roots in the IBA + myo-inositol combination treatments and shoot growth of MxM 14 explants (i.e. microcuttings height). The survival percentage of the ex vitro MxM 14 plantlets was 93 %. An efficient protocol of in vitro rooting and vegetative growth for the cherry rootstock MxM 14 was established using IBA and myo-inositol which constitutes a very useful tool for future genetic transformation.
Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
