Effects of exogenous amino acid applications on the plant-parasitic nematode Heterodera schachtii

Abstract

Summary Amino acid (AA) applications have been reported to affect plant-parasitic nematodes. Here, we analysed the effects of methionine (Met), lysine (Lys), threonine (Thr), isoleucine (Ile), homoserine (Hom) and tryptophan (Trp) on the sedentary plant-parasitic nematode, Heterodera schachtii, under in vitro conditions. No AA showed direct effects on the activity of infective second-stage juveniles (J2) of H. schachtii. Soaking J2 in Lys for 24 h increased the number of developing females and reduced the number of males. Thr treatments reduced the total number of nematodes developing in the host plant. The strongest effects were observed when AA were added to the nutrient medium in a monoxenic Arabidopsis thaliana culture. Ile, Met or Thr clearly reduced the number of female nematodes developing in the host plant. These AA are direct metabolic derivatives of Hom. Direct effects on pre-infective J2 can be differentiated from effects that may involve the host plant.