Effects Of Exercise On PPARγ Protein-protein Interaction In Subcutaneous Adipose Tissue Of High-fat Diet Rats

Abstract

PPARγ is an important transcription factor in regulating the lipid metabolism of adipose tissue in exercise. However, the PPARγ-centered network is still unclear. PURPOSE: To explore the effects of exercise on the PPARγ protein-protein interaction network (PPI) in subcutaneous adipose tissue of high-fat diet rats. METHODS:24 4-week-old male SD rats were randomly divided into standard quiet group (C), high-fat quiet group (HC), high-fat training group (HE), each of eight. Rats of the C group were fed a normal diet (D12450B), while HC and HE groups were fed a high-fat diet (D12451). Rats in each group were subjected to 8 weeks of the experimental intervention. HE group also received the exercise intervention for 5 days every week and underwent 60%-70% VO2max intensity treadmill training for 50 minutes per day. After an 8-week intervention, the body weight of the rats was weighed, the body composition was measured by DEXA scanning. 3 rats from each group who completed the intervention process were randomly selected for the PPI work of PPARγ in subcutaneous adipose tissue, which consists of COIP-MS experiment and bioinformatics analysis. RESULTS:1.After the intervention, there was no significant difference in body weight among the three groups. However, the body fat percentage in the HC group was significantly higher than in the C group (43.71 ± 9.06% vs. 36.36 ± 6.19%, P < 0.01). And the body fat percentage in the HE group was significantly lower than the HC group (35.34 ± 2.99% vs. 43.71 ± 9.06%, P < 0.01).2. The protein expression of PPARγ in group H was significantly reduced vs. group C (0.59 ± 0.16 vs. 1.00 ± 0.00, P < 0.05); the total number of differential proteins was 33, 11 were up-regulated and 22 were down-regulated. The expression of PPARγ in the HE group was significantly increased vs. group H (0.94 ± 0.13 vs. 0.59 ± 0.16, P < 0.05); the total number of differential proteins was 25, 6 were up-regulated, and 19 were down-regulated. CONCLUSIONS:1. The 8-week high-fat diet may inhibit the body's inflammatory response by down-regulating the expression of PPARγ and its direct interaction protein Crp, and regulate lipid metabolism.2.In the 8-week high-fat diet rat model under the intervention of exercise, the differential protein function is enriched in amino acid metabolism, etc., and the energy metabolism process is closely related.