Abstract
Objective To evaluate the effects of exendin-4 on glial brillary acidic protein （GFAP ） and interleukin-1β（IL-1β） expression in hippocampi of aged rats .Methods Forty-eight healthy male Sprague-Dawley rats ,aged 22-24 weeks ,weighing 500-700 g ,were randomly divided into 4 groups （n=12 each） using a random number table：control group （group C ） ,exendin-4 group （group E ） ,operation group （group O ） and exendin-4 plus operation group （group OE） .The rats were anesthetized with intraperitoneal fentanyl and droperidol .Groups C and E did not receive anesthesia or splenectomy .In O and OE groups ,splenectomy was carried out .In E and OE groups , exendin-4 5 μg/kg was injected intraperitoneally at 30 min before skin incision and 12 h after operation .C and O groups received the equal volume of normal saline instead of exendin-4 .Learning and memory function was assessed using Morris water maze test （escape latency （EL） and total swimming distance （TSD） at 1 day before operation （T0 ） .The fasting blood glucose was measured after anesthesia （T1 ） ,at the end of operation （T2 ） and on postoperative day 1 （T3 ） .The rats were sacrificed after assessment of the cognitive function at T 3 and the hippocampi were removed for determination of the expression of GFAP （by immuno-histochemistry ） and IL-1β（by Western blot ） .Results There was no significant difference in the EL and TSD at T0 between the four groups＆nbsp;（ P〉0.05） .Compared with group C ,the EL and TSD were significantly prolonged at T3 and fasting blood glucose was increased at T2 ,3 ,and the expression of IL-1βand GFAP was up-regulated at T3 in O and OE groups （ P〈0.05） .Compared with group O ,the EL and TSD were significantly prolonged at T3 and fasting blood glucose was decreased at T2 ,3 ,and the expression of IL-1βand GFAP was down-regulated at T3 in group OE （ P〈0.05） . Conclusion Exendin-4 can improve the postoperative cognitive function of aged rats by inhibiting inflammatory responses in hippocampi and maintaining stable perioperative blood glucose . Key words: Glucagon-like peptide 1; Cognition disorders; Hippocampus; Inflammation; Blood glucose
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