Effects of ethylenediaminetetraacetic acid on phytate phosphorus utilization and efficiency of microbial phytase in broiler chicks

Abstract

Previous studies have suggested that organic acids may improve P utilization in animals. To evaluate the ability of ethylenediaminetetraacetic acid (EDTA) to improve phytate P utilization and the possible synergistic effect between EDTA and microbial phytase (MP) an experiment was conducted using 360 Ross 308 broiler chicks. The experiment was carried out using a completely randomized design with a 3*2 factorial arrangement (0, 0.1 and 0.2% EDTA and 0 and 500 IU MP). Four replicate of 15 chicks per each were fed dietary treatments including (i) P-deficient basal diet [0.2% available phosphorus (aP)] (NC); (ii) NC + 500 IU MP per kilogram of diet; (iii) NC + 0.1% EDTA per kilogram of diet; (iv) NC + 0.1% EDTA and 500 IU MP per kilogram of diet; (v) NC + 0.2% EDTA per kilogram; and (vi) NC + 0.2% EDTA + 500 IU MP per kilogram of diet. Weight gain (WG), feed efficiency and serum Ca, P and alkaline phosphatase (ALP) were assessed. Addition of 0.2% EDTA to low aP diets resulted in significantly lower feed consumption (FC) and WG, but 0.1% EDTA did not depress WG compared to NC. Phytase supplementation of P-deficient diets significantly improved WG and feed efficiency, but it had no effect on FC. Microbial phytase supplementation significantly decreased ALP concentration. Results obtained in our study suggest no synergistic effect between phytase and EDTA in broiler chicks.