Abstract
Abstract The administration of dl-ethionine to female rats is followed by a decrease in hepatic nuclear ATP concentration which parallels the change in the whole liver. However, the magnitude of the nuclear ATP change is less than in the whole liver. The nucleus also shows a decrease in K+ and an increase in Na+ levels accompanying the changes in ATP. Nuclear Ca++ and Mg++ levels also decrease but much more slowly than does the ATP. The nuclear changes observed were all reversed by the administration of adenine several hours after the injection of ethionine. Liver microsomes show a transient increase in Mg++ concentration following ethionine administration. The possibility of separate adenosine-trapping phenomena in the nucleus and cytoplasm of the liver cell is discussed.
Highlights
If not all, RNA synthesis occurs in the nucleus, and since it is known that ethionine injection causes a large inhibition of RNA synthesis, it became important to determine whether the nucleus participates in the changes in ATP concentration induced in the whole liver by this methionine analogue
Such information might aid in the elucidation of the mechanism of inhibition of RNA synthesis by ethionine [5]
The RNA to DNA ratios, which are still higher than the ones obtained with rat liver nuclei isolated in an aqueous medium may be partially explained by a greater recovery, with organic solvents, of the ribosomes attached to the outer side of the nuclear envelope. That they reflect some cytoplasmic contamination of the organic solvent nuclei. That both the protein to DNA and RNA to DNA ratios tend to decrease as the time of ethionine treatment increases
Summary
The administration of DL-ethionine to female rats is followed by a decrease in hepatic nuclear ATP concentration which parallels the change in the whole liver. If not all, RNA synthesis occurs in the nucleus, and since it is known that ethionine injection causes a large inhibition of RNA synthesis, it became important to determine whether the nucleus participates in the changes in ATP concentration induced in the whole liver by this methionine analogue. Such information might aid in the elucidation of the mechanism of inhibition of RNA synthesis by ethionine [5]. The concentration of adenosine triphosphate in the liver (l-4), followed by a strikinginhibition of ribonucleic acid [5] and protein
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