Abstract
The effects of progesterone and/or 17β-estradiol on thymidine kinase activity and autoradiograms were investigated in immature rats. Thymidine kinase activity increased more than thirtyfold above the control level 30 hours after 17β-estradiol injection. The enzyme activity induced by 17β-estradiol was suppressed by progesterone, the dose of which was approximately 1,000-fold that of 17β-estradiol. The specific thymidine kinase isozyme, which was separated from 17β-estradiol-induced uterine thymidine kinase by diethylaminoethyl (DEAE) cellulose column chromatography and not affected by deoxycytidine triphosphate, was involved in the DNA replication and inhibited by progesterone. The autoradiogram revealed many grains due to 3H-thymidine in the endometrial epithelium, stroma, and the myometrium in the immature rat 30 hours after 17β-estradiol injection, whereas progesterone reduced remarkably the number of grains induced by 17β-estradiol in the epithelium. Progesterone seems to inhibit the increment of the specific thymidine kinase isozyme induced by 17β-estradiol in the endometrial epithelium.
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