Effects of Estradiol on Protein Synthesis in The Fibromuscular Stroma from the Guinea Pig Seminal Vesicle

Abstract

Estradiol treatment of intact and castrate adult male guinea pigs produced selective increases in the rate of [35S]methionine incorporation in vitro into 12,500 g soluble peptides of 58,000 and 97,000 Mr from the seminal vesicle fibromuscular stroma. Total incorporation into the 12,500 g soluble fraction was unchanged in estrogen-treated, intact animals. In castrate animals, the rate of total incorporation in the 12,500 g fraction was decreased by more than 50 per cent. Estrogen treatment of castrate animals restored the rate of total incorporation to that observed in untreated intact animals, indicating that estrogen was capable of maintaining normal levels of total incorporation into the 12,500 g soluble protein fraction. In castrate animals the administration of tamoxifen prevented the estrogen-induced maintenance of total [35S]methionine incorporation and inhibited the selective increase in incorporation into the 58,000 Mr peptide. From the results of this and other studies it may be suggested that estrogen modulates sex accessory stromal tissue function by altering the synthesis of specific proteins via an interaction with specific estrogen binding sites.