Effects of estradiol on in vitro maturation of buffalo and goat oocytes.

Abstract

PurposeThe effects of estradiol on oocyte development seem to be varied among species. The present study investigated the effects of 17β‐estradiol on in vitro maturation of buffalo and goat oocytes.MethodsCumulus oocyte complexes (COCs) were aspirated from large antral follicles of slaughtered buffalo and goat ovaries. COCs were cultured in TCM‐199 medium supplemented with 0, 0.5, 1, and 1.5 µg/mL of 17β‐estradiol for in vitro maturation. Then, oocytes were used for the examination of state of nuclear maturation and cumulus expansion.ResultsIn both species, oocytes treated with 17β‐estradiol showed higher cumulus expansion rate than control (0 µg/mL treated). In buffalo, the percentage of oocytes matured to the metaphase II (MII) stage increased in the concentration‐dependent manner of 17β‐estradiol. Similarly, estradiol positively influenced nuclear maturation of goat oocytes in vitro.ConclusionsEstradiol has promoting effects on normalprogress of in vitro oocyte meiosis in buffalos and goats.