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Abstract
Effects of estradiol on skeletal tissue calcification were examined in the goldfish, Carassius auratus. The fish were given estradiol-17β and placed in a tank containing 45Ca. 45Ca deposition on the vertebra, rib and otolith was examined with a gas flow counter. The calcium content of the tissues was determined with atomic absorption spectrophotometry. Plasma calcium was also analysed to monitor the effect of the hormone. 1. A single injection of estradiol resulted in marked hypercalcemia. 2. Calcium deposition on the vertebra and the rib was significantly inhibited by 43% and 38%, respectively, at an early stage of estrogenization. 3. No effect was found in the otolith. 4. Calcium content of the tissues remained unchanged following estrogenization.
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