Effects of er-miao-san extracts on TNF-alpha-induced MMP-1 expression in human dermal fibroblasts.

Seunghee Bae,Shunhua Li,Younjung Jung,Yeong Choi

doi:10.1186/0717-6287-48-8

Seunghee Bae, Shunhua Li + Show 2 more

Open Access

https://doi.org/10.1186/0717-6287-48-8

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Journal: Biological research	Publication Date: Jan 1, 2015
Citations: 46	License type: cc-by

Affiliation: Konkuk University

Abstract

BackgroundVarious health benefits have been attributed to Er-Miao-San (EMS), a traditional Chinese herbal formulation that contains equal amounts of cortex phellodendri (Phellodendron amurense Ruprecht) and rhizoma atractylodis (Atractylodes lancea D.C). However, its effect on the anti-inflammatory activity in human dermal fibroblasts (HDFs) and the mechanism underlying this effect are unknown.ResultsThis study investigated the effects of EMS on TNF-α-induced MMP-1 expression in HDFs. Our data show that EMS inhibited TNF-α-induced MMP-1 expression in a concentration-dependent manner. Interestingly, EMS maintained IκB content without inhibiting the phosphorylation of MAPKs, which are well-established upstream kinases of NF-κB. Moreover, EMS reduced the level of nuclear p65 protein in HDFs. Luciferase assay revealed that EMS inhibits the transcriptional activity of NF-κB by stabilizing IκB. Our results show that EMS exerts its anti-inflammatory effect by inhibiting NF-κB-regulated genes such as IL-1β and IL-8. Moreover, EMS effectively inhibited TNF-α-induced expression of MMP-1 via the NF-κB pathway.ConclusionsTaken together, our data suggest that EMS could potentially be used as an anti-inflammatory and anti-aging treatment.

Highlights

Various health benefits have been attributed to Er-Miao-San (EMS), a traditional Chinese herbal formulation that contains equal amounts of cortex phellodendri (Phellodendron amurense Ruprecht) and rhizoma atractylodis (Atractylodes lancea D.C)
Effect of EMS on cell viability We first investigated whether the treatments of EMS, Cortex Phellodendri (CP) and Rhizoma Atractylodis (RA) on human skin dermal fibroblasts will induce cytotoxic or non-cytotoxic properties, and what are the non-cytotoxic concentration ranges of EMS, CP and RA on human skin dermal fibroblasts before further experiments that investigate the EMS-induced protection effect on TNF-α-induced matrix metalloproteinase (MMP)-1 expression
Effect of EMS on TNF-α-induced expression of MMP-1 mRNA We investigated whether TNF-α-induced downregulation of MMP1 expression could be regulated by treating EMS in human dermal fibroblasts (HDFs)

Summary

Introduction

Various health benefits have been attributed to Er-Miao-San (EMS), a traditional Chinese herbal formulation that contains equal amounts of cortex phellodendri (Phellodendron amurense Ruprecht) and rhizoma atractylodis (Atractylodes lancea D.C). Its effect on the anti-inflammatory activity in human dermal fibroblasts (HDFs) and the mechanism underlying this effect are unknown. Oxidative stress due to UVB, an extrinsic aging factor, causes DNA damage, and leads to human dermal fibroblast (HDF) senescence [2]. Following the degradation of IκB, NF-κB translocates to the nucleus and induces transcription of various inflammatory genes, including interleukin (IL)-1β and IL-8 [11,12]. Accumulating studies have shown that NF-κB regulates skin aging by regulating the expression level of MMP-1 in dermal fibroblasts [13,14]. Inflammation-induced activation of NF-κB causes deterioration of dermal tissue by promoting the expression of MMP-1, which exerts degradation of dermal type I collagen [16]

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