Abstract
Objectives To explore effects of Epstein-Barr virus (EBV) infection on CD19+ B lymphocytes in patients with immunorelated pancytopenia (IRP). Methods An enzyme-linked immunosorbent assay (ELISA) in vitro diagnostic kit was used to detect EBV capsid antigen- (CA-) IgG and VCA-IgM antibodies in the serum. We analyzed the EBV-DNA copies of CD19+ B lymphocyte by using real-time quantitative polymerase chain reaction (RT-qPCR). CD21, CD23, CD5, CD80, and CD86 receptors on the surfaces of CD19+ B cells were detected by flow cytometry (FCM). The correlation between these receptors and EBV-DNA copies were evaluated. Results The results revealed that the positive rate of EBVCA-IgM and CD19+ B lymphocyte EBV-DNA copy in the IRP group were significantly higher than those in the control group (P < 0.05). CD19+ B lymphocyte EBV-DNA copies were also more abundant in IRP patients than in control subjects (P < 0.05). CD19+ B lymphocyte EBV-DNA copies were also more abundant in IRP patients than in control subjects (P < 0.05). CD19+ B lymphocyte EBV-DNA copies were also more abundant in IRP patients than in control subjects (Conclusions EBV infection may activate CD19+ B lymphocytes and further disrupt bone marrow hematopoiesis in IRP patients.
Highlights
Immunorelated pancytopenia (IRP) is a type of hemocytopenia regarded as an autoimmune disease that is caused by unknown autoantibodies, which may suppress hematopoietic cells in the bone marrow, leading to anemia, bleeding, and infection [1]
EBVCA-IgMpositive rate was significantly higher in IRP patients than in control subjects (χ2 = 4:515, P = 0:033; Table 1)
Cases exhibiting persistent hemocytopenia not attributable to hematological or nonhematological diseases have been described as idiopathic cytopenia of EB-DNA load
Summary
Immunorelated pancytopenia (IRP) is a type of hemocytopenia regarded as an autoimmune disease that is caused by unknown autoantibodies, which may suppress hematopoietic cells in the bone marrow, leading to anemia, bleeding, and infection [1]. IRP pathogenesis is considered to result from abnormalities in the number, subsets, function, and activation of B lymphocytes [5]. The Epstein-Barr virus (EBV) belongs to a class of viruses with double-stranded DNA that are hosted by B lymphocytes. These viruses can interfere with immune function and stimulate cell proliferation and transformation [6, 7]. EBV is thought to be an environmental trigger of, and one of the principal candidates that causes, autoimmune diseases. EBV is associated with autoimmune diseases such as systemic lupus erythematosus (SLE), rheumatoid arthritis (RA), multiple sclerosis (MS), autoimmune thyroiditis, inflammatory bowel disease, insulin-dependent diabetes mellitus, Sjögren’s syndrome, systemic sclerosis, myasthenia gravis, and autoimmune liver disease [8]
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