Effects of epicatechin gallate (ECG) on fetal bovine serum (FBS)-induced steatosis in human liver cell line L02 and 2,2′-azobis (2-amidinopropane) (AAPH)-induced oxidative stress in human erythrocytes

Abstract

In this research, 2,2′-azobis (2-amidinopropane)-induced oxidative stress in human erythrocytes and 50 % fetal bovine serum-induced steatosis in human liver L02 cell were used to evaluate the functions of epicatechin gallate (ECG) in regulation of lipid metabolism and inhibition of oxidative stress. The results indicated that the antioxidant rationales of ECG in biological systems mainly involved in suppressing the generation of reactive oxygen species by preserving the antioxidant enzyme activities of (superoxide dismutase and glutathione peroxidase) and reducing the consumption of reduced glutathione as well as inhibiting the production of thiobarbituric acid-reactive substances. The anti-nonalcoholic fatty liver disease rationales of ECG included inhibiting the growth of steatosis L02 cells, reducing the accumulation of hepatic lipid through lowering the levels of total cholesterol and triacylglycerol, and suppressing the leakage volume of enzymes (aspartate aminotransferase, alanine aminotransferase and lactate dehydrogenase).