Effects of enucleation method on in vitro and in vivo development rate of cloned pig embryos

Chengcheng Zhao,Dewu Liu,Enqin Zheng,Gengyuan Cai,Hongmei Ji,Junsong Shi,Lvhua Luo,Ranbiao Mai,Rong Zhou,Xiaoyan He,Zhenfang Wu,Zicong Li

doi:10.15302/j-fase-2018227

Abstract

Effects of enucleation method on in vitro and in vivo development rate of cloned pig embryos

Highlights

Enucleation is a key step for successful somatic cell nuclear transfer (SCNT)
Sows that received BAB-generated SCNT embryos exhibited the same pregnancy rates (69.70% vs. 69.70%) and farrowing rates (42.42% vs. 42.42%) as sows that received spindle imaging system-assisted (SIS-A)-generated SCNT embryos (Table 3)
The results of the current study indicated that SIS-A enucleation achieves a higher number of successfully enucleated oocytes and tends to produce a higher in vivo SCNT embryo development rate than blind aspiration-based (BA-B) enucleation

Summary

Introduction

Enucleation is a key step for successful somatic cell nuclear transfer (SCNT). It is important especially in the Received December 7, 2017; accepted February 24, 2018Blind aspiration-based (BA-B) enucleation is a simple mechanical technique commonly used in SCNT. Enucleation is a key step for successful somatic cell nuclear transfer (SCNT). It is important especially in the Received December 7, 2017; accepted February 24, 2018. Blind aspiration-based (BA-B) enucleation is a simple mechanical technique commonly used in SCNT This method performs enucleation by removing a small volume of cytoplasm that presumably contains the nucleus adjacent to the first polar body[1,2]. Spindle imaging system-assisted (SIS-A) enucleation has been used for oocyte enucleation in pig, cattle and other species[6,7,8,9,10,11,12,13] This method employs a polarized light microscope to visualize the meiotic spindle-containing oocyte nucleus

Methods

Results

Conclusion