Effects of endurance training on hsa-miR-223, P2RY12 receptor expression and platelet function in type 2 diabetic patients.

Abstract

Type 2 diabetes mellitus (T2DM) is associated with thrombotic events due to platelets' defects. The aim of this study was to investigate the effects of endurance training on expressions of platelet hsa-miR-223 and P2RY12 receptor, as well as platelet function in T2DM patients. Twenty female T2DM patients (age, 62.25±3.81 yr; weight, 73.53±9.04 kg; height, 154.7±4.3 cm) were randomly divided into two groups (Control (CONT) and endurance training (ET)). Subjects in ET group performed eight weeks of running on treadmill at 60-75% of VO2peak (moderate intensity), 3 sessions per week, while, the subjects in control group were not involved in any kind of exercise training. Two blood samples were taken before and 48 h after the training and were analyzed for platelet aggregation, and hsa-miR-223 and P2RY12 expressions. Although platelet aggregation decreased significantly in ET group (P < 0.05), these changes were not significantly different between two groups. Expression of platelet hsa-miR-223 increased and P2RY12 mRNA reduced following ET non-significantly. However, decreases in fasting blood glucose, glycated hemoglobin and body weight, and increases in VO2peak following ET were significantly different when compared to control group (P < 0.05). Short-term endurance training dose not induce up-regulation of hsa-miR-223 and down-regulation of P2RY12, while it has a positive impact on platelet function, glycemic indices, physical fitness and body composition in female T2DM patients.