Effects of endotoxin on glycogenolytic enzymes of mouse liver.

Abstract

SummaryThe effect of endotoxin on the glycogenolytic enzymes was determined in an in vitro system. No increases in activities of phosphorylase, acid lysosomal glucosidase, neutral glucosidase or amylo-1, 6-glucosidase were noted. The increased glycogenolysis was attributed to increased activity of α-amylase, which resulted in release of α-amylase from microsomes. Endotoxin did not release acid phosphatase from lysosomes suggesting that release of microsomal α-amylase was not mediated by released lysosomal enzymes. Preliminary experiments did not show inhibition of microsomal membrane-bound ATPase.The livers of mice injected intravenously with endotoxin had one-sixth of the micro-somal-associated α-amylase activity as compared with control mice, indicating in vivo microsomal damage. The sera of these endo-toxin-treated mice had a corresponding increase in α-amylase activity over the sera of control mice.This research was supported by National Science Foundation Grant GB-8363. P. Zwadyk was the recipient of...