Effects of Emulsion Formulations of Oleuropein Isolated from Ethanol Extract of Olive Leaf in Diabetic Rats.

Ahmet G Aggul,Emrah Ozakar,Mine Gulaboglu,Meltem Cetin,Tuba Aydin,Rukiye S Ozakar

doi:10.1590/0001-3765202020190810

Abstract

This study was designed to investigate the effects of emulsion formulations of oleuropein isolated from ethanol extract of olive leaf in streptozotocin-diabetic rats. The rats were treated with the administration of the emulsion containing oleuropein at a low (150 mg/kg b.wt.) and high (225 mg/kg b.wt.) dose for 30 days. At the end of the study, blood samples were drawn from the heart of the rats to determine blood glucose, alanine transaminase, and aspartate transaminase levels. In addition, their liver tissues were dissected to determine the levels of glutathione and thiobarbituric acid-reactive substances, and superoxide dismutase activity. According to the results for both dose treatments, a statistically significant increase in superoxide dismutase activities and glutathione levels of the treated diabetic rats was observed when compared with those of the diabetic control rats. On the other hand, a statistically significant decrease in the levels of thiobarbituric acid-reactive substances, aspartate transaminase and alanine transaminase of the treated diabetic rats was determined. It should be highlighted that the administrations at the high dose were more effective compared to that of the low dose. Furthermore, a substantial decrease in the blood glucose levels of the diabetic rats exposed to the high dose was observed.

Highlights

Diabetes Mellitus (DM), a disease requiring continuous medical care for the patients, is a chronic metabolic disease that has high mortality and morbidity rates in both developed and developing countries
The STZ-induced diabetic rats were treated with the administrations of the solutions by oral gavage
Reports of the experimental studies of OL, a natural antioxidant compound, have been published in 2006 (Al-Azzawie & Alhamdani 2006) and 2009 (Jemai et al 2009). These confirmed antioxidant properties allow OL to be efficient in the protection against some metabolic diseases related to oxidative stress such as diabetes

Summary

Introduction

Diabetes Mellitus (DM), a disease requiring continuous medical care for the patients, is a chronic metabolic disease that has high mortality and morbidity rates in both developed and developing countries. In diabetes, uncontrolled increased blood glucose can lead to producing overwhelming free radicals. Diabetes is generally accompanied by a chronic state of oxidative stress (Wu et al 2018, Eidi et al 2009, Jemai et al 2009). Oxidative stress associated with diabetes has an increasing effect on lipid peroxidation (Davi et al 2005) and hepatic enzyme levels (Eidi et al 2009). It has a decreasing effect on antioxidant enzyme activities (Jemai et al 2009, Al-Azzawie & Alhamdani 2006) and changes glutathione redox status in diabetic patients

Methods

Results

Discussion

Conclusion