Abstract
The effects of emulsified perfluorochemicals (PFCs) and some of their constituents on growth and ultrastructure of microbial cells in culture have been studied. Growth rate ofE. coli was inhibited by stem emulsion from the proprietary formulation, Fluosol-DA 20%, and also by a combination of the Pluronic F-68 surfactant and yolk phospholipid emulsion stabilizer. Yeast growth was also inhibited by Fluosol stem emulsion and by pluronic alone. Electron microscopical examination of thin sections of yeast cells following culture in perfluorodecalin emulsion revealed cytoplasmic vacuolation and other ultrastructural perturbations resembling those reported previously in mammalian cells cultured with emulsified PFCs; no comparable structural changes inE. coli were observed.
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