Effects of elevated temperatures and cadmium exposure on stress biomarkers at different biological complexity levels in Eisenia fetida earthworms

Abstract

Several ecotoxicological studies assessed metal toxicity upon soil biota and other communities but were mainly focused on the study of a single chemical and usually under optimal conditions of temperature. Meanwhile an increasing global warming is leading to new scenarios by combining different stress factors; chemical stress and thermal stress. Presently, this study aims to assess the joint effects produced by cadmium and elevated temperature on earthworms different levels of biological complexity. Eisenia fetida earthworms were maintained at 19 °C and 26 °C and simultaneously exposed to four Cd concentrations (1.25, 2.5, 25 and 125 mg Cd/Kg soil) for 14 (Short term exposure) and 56 days (reproduction test). Endpoints were addressed at different levels of biological complexity: reproductive impairment (cocoons and juvenile productions), Cd tissue accumulation, mortality of adults, weight loss and cytotoxic effects (coelomocyte viability). In the Short term exposure, increase in temperature produced a larger accumulation of Cd. Hence, earthworms exposed to 125 mg Cd/kg soil under heat stress (26 °C) showed a two fold higher Cd accumulation comparing to those at 19 °C. Earthworms exposed to moderate-high concentrations of Cd (2.5–125 mg Cd/kg) and maintained at 26 °C showed severe weight loss and high mortality rates. The neutral red uptake capacity of coelomocytes extruded from earthworms exposed to the highest Cd concentration decreased after 14 d at 19 °C, and more markedly at 26 °C. The reproduction impairment (decreased number of cocoons) was enhanced after exposure to concentrations higher than 2.5 mg Cd/kg at 26 °C, and after exposure to 125 mg Cd/kg at 19 °C. Earthworm reproduction capability is highly vulnerable to the effect of toxicants at elevated temperatures and sublethal concentrations.