Effects of Elevated Surface Ozone Concentration on Photosynthetic Fluorescence Characteristics and Yield of Soybean Parents and Offspring

Abstract

Global climate change presents a significant threat to food security. Analyzing the effects of elevated ozone (O3) concentration on photosynthetic fluorescence characteristics and yield addresses the damage of climate change on crops, which would serve food security. With open-top chambers (OTCs) and Tiefeng-29 soybeans, we investigated the responses of chlorophyll concentration, fluorescence characteristics, net photosynthetic rate (Pn) and yield components to different O3 concentrations, which included CK (ambient concentration approximately 45 nL·L−1, T1 (80 ± 10) nL·L−1 and T2 (120 ± 10) nL·L−1 O3. The parent soybeans (S1) were planted in the current year, and O3 fumigation commenced 20 days after seedling emergence. Aeration was stopped at maturity, and the offspring soybeans (S2) were retained after harvest for further experiments. In the following year, S1 and S2 soybeans were planted, and O3 fumigation began 20 days after seedling emergence. The results show that leaf chlorophyll a (chla) and chlorophyll b (chlb) significantly decreased with longer O3 fumigation time both in parents and offspring, causing damage to the light-trapping ability while the offspring suffered an earlier decrease. The elevated O3 damaged the electron transfer process by significantly reducing the original and actual photochemical efficiencies of PSII both in parents and offspring. The electron transfer rate (ETR) of the parents and offspring decreased, while the difference between them was not significant after O3 treatment. The non-photochemical quenching coefficient (NPQ) showed an increasing trend along time but showed no significant difference between parents and offspring. An elevated concentration of O3 significantly reduced Pn, while the differences in Pn between the parents and the offspring were not significant. Elevated O3 resulted in reduced yields in both parent and offspring soybeans. Although it was found that the offspring soybeans exhibited higher yields than the parents, their reduction in yield was more significant. Therefore, elevated O3 concentration reduced soybean yield through damaging photosynthetic process and electron transfer capacity by impairing energy conversion and material accumulation capacity. The offspring had relatively higher light energy conversion efficiency than the parents, resulting in a higher yield than the parents under all treatments.